[Characteristics of asparaginase from Escherichia coli at different stages of purification]

Asparaginase of Escherichia coli obtained according to the previously developed scheme (extraction, acidification, heating with ammonium sulphate, twice repeated precipitation with ethanol and DEAE-cellulose chromatography) was examined by the methods of polyacrylamide gel electrophoresis, isoelectric focusing and molecular weight assay. Asparaginase activity was measured by the Nessler method. With respect to the above characteristics, the asparaginase preparation was very similar to those described in the literature, e.g. Krasnitin (FRG) and Leunase (Japan).