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Sperm-immunobead binding decreases with in vitro incubation
J E Gould1, C K Brazil, J W Overstreet
1University of California, School of Medicine, Davis.
Objective:
To characterize the stability of the sperm-immunobead association over time.
Design:
Prospective evaluation of sperm-immunobead binding, using direct and indirect assays.
Setting:
Male Infertility Clinic, University of California, Davis, Davis, California.
Patients:
Eleven men with sperm surface antibodies and 25 men with serum antisperm antibodies volunteered.
Main Outcome Measures:
Repeated assessment of sperm-immunobead binding over time.
Results:
Serum immunoglobulin (Ig)G decreased a mean of 42.6% over 30 minutes, and serum IgA decreased a mean of 22.7% over 30 minutes. Semen-derived IgG binding fell a mean of 59.9% and semen-derived IgA fell a mean of 27.0% over 25 to 40 minutes.
Conclusions:
Sperm-immunobead coincubation results in a decrease in the number of sperm bound to immunobeads.