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High-performance liquid chromatographic method for the separation of chlorambucil and its N-oxide prodrug
K J Chandler1, J B McCabe, D L Kirkpatrick
1Department of Chemistry, University of Regina, Sask, Canada.
Journal of Chromatography
|February 11, 1994
Abstract:
A reversed-phase high-performance liquid chromatographic method is described to distinguish chlorambucil N-oxide from the parent chlorambucil and quantitate both after separation from biological samples. The influence of solvent pH, alcohol, acid and ion-pairing agent on the separation is described. The stability of chlorambucil and its N-oxide in buffers and alcohols, as well as stability during filtration is discussed with potential application for metabolic studies.