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Polyhydroxamic microcapsules prepared from proteins: a novel type of chelating microcapsules
D Hettler1, M C Andry, M C Levy
1Laboratoire de Pharmacotechnie, URA/CNRS 492, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, France.
Journal of Microencapsulation
|March 1, 1994
Summary
Modified protein microcapsules demonstrate enhanced iron binding capabilities after alkaline hydroxylamine treatment. This modification improves their chelating properties, making them suitable for iron sequestration applications.
Area of Science:
- Materials Science
- Biochemistry
- Chemical Engineering
Background:
- Protein-based microcapsules are investigated for various applications.
- Interfacial crosslinking is a common method for microcapsule fabrication.
- Modification of microcapsule surfaces can alter their functional properties.
Purpose of the Study:
- To modify protein microcapsules (HSA, fibrinogen, ovalbumin) for enhanced iron binding.
- To investigate the effect of alkaline hydroxylamine treatment on microcapsule properties.
- To assess the impact of esterification on iron chelating capacity.
Main Methods:
- Microcapsule preparation via interfacial crosslinking with terephthaloyl chloride.
- Alkaline hydroxylamine treatment to disrupt bonds and introduce hydroxamic groups.
- Iron binding capacity assessment using ferric chloride solutions.
- Esterification of carboxyl groups using carbodiimide.
Main Results:
- All microcapsules survived hydroxylamine treatment, increasing in size and becoming trypsin-sensitive.
- Hydroxylamine treatment imparted iron-binding capabilities to the microcapsules.
- Human serum albumin (HSA) microcapsules showed lower iron binding (29.3 µmol/g) compared to fibrinogen and ovalbumin (43.7-44.9 µmol/g).
- Esterification of carboxyl groups significantly improved iron binding to approximately 50 µmol/g for all protein types.
Conclusions:
- Alkaline hydroxylamine treatment effectively modifies protein microcapsules for iron binding.
- Esterification further enhances the iron chelating efficiency of these modified microcapsules.
- These modified microcapsules show potential for applications requiring iron sequestration.