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Updated: Aug 12, 2026

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
Published on: June 9, 2015
[Studies on culturing seeds of Tripterygium hypoglaucum (Level.) Hutch. in vitro]
Abstract:
This paper for the first time reports the embryo culturing in vitro of Tripterygium hypoglaucum. Tests were conducted of every condition for the normal germination and growth of the excised embryo. The result shows that the germination ratio reaches the highest on the white basic medium when the intensity of light is 400Lx, sucrose concentration is 3% and whole nitrogen content is 230 mg/L. Intact plantlets are obtained from the aseptic shoots. A method using aseptic shoots to accelerate the propagation of plantlets is found primarily.
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