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Perfusion systems for hybridoma cells based on sedimentation in chambers and Erlenmeyer flasks
1Department of Biotechnology, Technical University of Denmark, Lyngby.
FEMS Microbiology Reviews
|May 1, 1994
Abstract:
An inclined sedimentation chamber and a modified 250-ml Erlenmeyer flask have been used as separation devices for perfusion fermentations with hybridoma cells. The maximum cell density is increased 2-16-fold compared to batch fermentations when the separation units are used. When the sedimentation chamber is used, IgG is continuously produced and the daily production is increased by a factor 3.7 compared to batch fermentation.