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Simple and rapid determination of phytase activity
A J Engelen1, F C van der Heeft, P H Randsdorp
1Gist-brocades b.v., Intracompany Service Laboratory, Delft, The Netherlands.
Journal of AOAC International
|May 1, 1994
Summary
This study presents a simple and rapid method for measuring microbial phytase enzyme activity. The technique quantizes inorganic orthophosphate released from sodium phytate hydrolysis.
Area of Science:
- Biochemistry
- Enzymology
- Microbiology
Background:
- Microbial phytase plays a crucial role in phosphorus metabolism.
- Accurate determination of phytase activity is essential for various applications.
- Existing methods for phytase activity assay can be complex or time-consuming.
Purpose of the Study:
- To develop a simple and rapid method for quantifying microbial phytase enzymatic activity.
- To establish a reliable assay for phytase based on inorganic phosphate release.
Main Methods:
- The method involves the enzymatic hydrolysis of sodium phytate.
- Inorganic orthophosphate released is quantified spectrophotometrically.
- The assay is optimized and validated at pH 5.5.
Main Results:
- The described method provides a straightforward and quick determination of microbial phytase activity.
- The assay demonstrates sensitivity and reliability in measuring phytase-derived inorganic phosphate.
- The method is applicable under specific conditions (pH 5.5).
Conclusions:
- A simple, rapid, and reliable method for assessing microbial phytase activity has been established.
- This assay facilitates efficient screening and characterization of microbial phytase enzymes.
- The method's simplicity makes it accessible for routine laboratory use.