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Published on: July 19, 2011
A method of demonstrating intra-renal reflux
1Department of Anatomy, University of Cambridge, UK.
Objective:
To develop a method for demonstrating intrarenal reflux as a permanent resin cast, and subsequently to show the relationship between the shape of the papilla and the presence and extent of reflux and to determine, by histological examination of the resin casts, the extent of filling of the nephron.
Subjects And Methods:
The pelvicaliceal systems of fresh pig's kidneys were filled retrogradely with a dilute resin that sets within 2 h. The parenchyma was then macerated in potassium hydroxide to obtain the casts.
Results:
The extent of the intra-renal reflux was variable, was usually polar and appeared independent of the injection pressure. Microscopy showed branching of the collecting ducts and nephron filling to the proximal tubules.
Conclusion:
This method could prove a useful tool in the further study of intra-renal reflux.
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