Related Experiment Videos
[Tests of Helicobacter pylori survival in milk]
S Kafel1, E Pogorzelska, A Lekowska-Kochaniak
1Kliniki Chorób Metabolicznych i Gastroenterologii Instytutu Zywności i Zywienia, Warszawie.
Abstract:
The survival of microaerophilic bacteria Helicobacter pylori (Hp) isolated from gastric mucosa was studied in 2 ml and 10 ml samples of milk contaminated with a suspension of these bacteria in 20% glucose solution. Milk was kept at 5-7 degrees C temperature in refrigerator and at room temperature. Hp was found in samples kept at 5-7 degrees C during 3 days. On the other hand, Hp was not found after 24-hours keeping at room temperature.
Insights
Helicobacter pylori (Hp) survived in milk for 3 days at 5-7°C but not after 24 hours at room temperature. This study highlights temperature's critical role in Hp survival in dairy products.
Area of Science:
- Microbiology
- Food safety
- Bacteriology
Background:
- Helicobacter pylori (Hp) is a microaerophilic bacterium commonly associated with gastric infections.
- Understanding the survival of Hp in food matrices is crucial for public health and food safety.
- Milk is a common food product susceptible to microbial contamination.
Purpose of the Study:
- To investigate the survival rate of Helicobacter pylori (Hp) in milk under different temperature conditions.
- To determine the impact of storage temperature on the viability of Hp in dairy products.
Main Methods:
- Samples of milk (2 ml and 10 ml) were artificially contaminated with a suspension of Helicobacter pylori (Hp) in 20% glucose solution.
- Contaminated milk samples were stored at two different temperatures: 5-7°C (refrigerator) and room temperature.
- Bacterial survival was assessed over time by detecting the presence of Hp.
Main Results:
- Helicobacter pylori (Hp) remained viable in milk samples stored at 5-7°C for up to 3 days.
- In contrast, Hp was not detected in milk samples kept at room temperature after 24 hours of storage.
- The volume of the milk sample (2 ml vs 10 ml) did not appear to significantly affect Hp survival within the tested timeframe.
Conclusions:
- Refrigeration temperatures (5-7°C) significantly extend the survival of Helicobacter pylori (Hp) in milk compared to room temperature.
- Room temperature poses a rapid inactivation risk for Helicobacter pylori (Hp) in milk, suggesting reduced transmission risk under such conditions.
- These findings have implications for food handling practices and risk assessment of dairy products regarding Hp contamination.