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[Use of rabbit polyclonal antibodies to human annexins for recognition of inherent proteins]
Rabbit antibodies generated against annexins of human origin recognize a variety of rabbit own annexins. The antibodies under study can be divided into two groups. The first group comprises antibodies against human annexins I and IV-VI which are sufficiently specific and show only slight cross-reactivities with other proteins, whereas the second group includes antibodies against human annexins I and III which react with many different annexins and in this relation resemble consensus peptide antibodies. Antibodies of either kind can be used for identifying rabbit own annexins. This eliminates the necessity to use other laboratory animals for immunization.
Rabbit antibodies generated against annexins of human origin recognize a variety of rabbit own annexins. The antibodies under study can be divided into two groups. The first group comprises antibodies against human annexins I and IV-VI which are sufficiently specific and show only slight cross-reactivities with other proteins, whereas the second group includes antibodies against human annexins I and III which react with many different annexins and in this relation resemble consensus peptide antibodies. Antibodies of either kind can be used for identifying rabbit own annexins. This eliminates the necessity to use other laboratory animals for immunization.