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Mechanisms Underlying Gut Hormone Secretion Using the Isolated Perfused Rat Small Intestine
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Mechanisms Underlying Gut Hormone Secretion Using the Isolated Perfused Rat Small Intestine

Published on: February 26, 2019

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Ontogenic regulation of phospholipase C-gamma 1 activity and expression in the rat small intestine

D B Polk1

  • 1Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee.

Gastroenterology
|July 1, 1994
PubMed

Insights

During weaning, rat intestinal phospholipase C-gamma 1 (PLC gamma 1) expression and activity increase, showing age-dependent regulation by epidermal growth factor (EGF). This study demonstrates in vivo EGF-dependent PLC gamma 1 tyrosine phosphorylation.

Area of Science:

  • Developmental biology
  • Molecular biology
  • Gastroenterology

Background:

  • The postnatal rat small intestine undergoes significant developmental changes during weaning.
  • Phospholipase C-gamma 1 (PLC gamma 1) is a key enzyme in growth and development pathways, acting as a substrate for the epidermal growth factor receptor (EGFR).

Purpose of the Study:

  • To investigate developmental changes in intestinal PLC gamma 1 expression, catalytic activity, and its regulation by growth factors.
  • To define the role of PLC gamma 1 in intestinal development during the weaning period.

Main Methods:

  • Immunodetection was employed to assess the expression and tyrosine phosphorylation of PLC gamma 1, EGFR, PI 3-kinase, GAP, and SHC in postnatal rat intestines.
  • Comparative analysis was performed between suckling, weanling, and adult animals.

Main Results:

  • Both the catalytic activity and expression of PLC gamma 1 significantly increased in the rat intestine during weaning.
  • Epidermal growth factor (EGF) stimulation led to increased PLC gamma 1 activity and tyrosine phosphorylation in weanling animals, but not in suckling animals.
  • Expression of EGFR and SHC was elevated in weanling rats compared to suckling and adult rats.

Conclusions:

  • Intestinal PLC gamma 1 expression and catalytic activity peak during the weaning period in rats.
  • EGF-dependent regulation of PLC gamma 1 tyrosine phosphorylation and activity is modulated by age.
  • This study provides the first in vivo evidence of EGF-dependent PLC gamma 1 tyrosine phosphorylation in normal animal tissue.
Abstract

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