A reliable method of embedding a small amount of dispersed cells for electron microscopy

To embed a small amount of dispersed cells for electron microscopy, we used the supernatant of agarose solution which had been centrifuged beforehand. We also warmed the chamber of the centrifuge by placing a flexible ribbon heater at the bottom of the chamber to prevent the premature gelation of agarose. By these methods, as few as 10(6) dispersed cells can be reproducibly processed and embedded for electron microscopy.