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Development of an RNA/RNA hybridization assay for the detection of the HAV CF53 strain
H van Cuyck-Gandré1, T Livache, J Ringeaud
1Unité de Biologie Moléculaire, Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Research in Virology
|January 1, 1994
Abstract:
A quick and sensitive dot-blot assay using non-radioactive labelled RNA probes was developed for the detection of the CF53 strain of hepatitis A virus (HAV) in cell culture. The cDNA of the 5' end of the HM175 strain was inserted in a transcription vector pSPT18 and was used to synthesize 32P- or digoxigenin-labelled RNA probes. These RNA probes specifically detected the RNA of the CF53 strain and can be used to detect HAV in PLC/PRF/5 cells. The sensitivity of non-radioactive tests was comparable to that of radiolabelled probes.