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Immunoglobulin formation in B lymphoid cells

B A Askonas

    Journal of Clinical Pathology. Supplement (Association of Clinical Pathologists)
    |January 1, 1975
    PubMed
    Summary
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    Mature plasma cells secrete large amounts of immunoglobulin (Ig), while small B lymphocytes produce less Ig, primarily for cell surface receptors. Antigen stimulation triggers B cell maturation into Ig-secreting cells, with a 24-hour lag in Ig production.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Biology

    Background:

    • Mature plasma cells are known for high immunoglobulin (Ig) secretion.
    • Ig synthesis involves light (L) and heavy (H) chain formation and assembly into 7S molecules (H2L2).
    • Polymeric Ig (IgM, IgA) form via disulfide bond interchange and J chain incorporation during secretion.

    Purpose of the Study:

    • To contrast Ig synthesis patterns between mature plasma cells and small B lymphocytes.
    • To investigate the regulatory mechanisms and intracellular events during B cell maturation into Ig-secreting cells.
    • To analyze Ig synthesis in normal lymphoid tissues, moving beyond studies on malignant myeloma cells.

    Main Methods:

    • Comparative analysis of Ig synthesis in mature plasma cells versus small B lymphocytes.

    Related Experiment Videos

  • Examination of Ig chain formation, assembly, and secretion processes.
  • Preliminary investigation of intracellular events in lipopolysaccharide-stimulated mouse spleen cells, focusing on mRNA transcription and translation.

    • Main Results:

    • Small B lymphocytes produce significantly less Ig than mature plasma cells, with most Ig associated with the cell surface as receptors.
    • Surface Ig receptors on IgM-producing cells exhibit slow turnover (24-48 hours) and are not actively secreted.
    • Antigen and cell-cell interactions stimulate B cell division and maturation.
    • Preliminary data suggest H-chain mRNA transcription mirrors DNA synthesis kinetics in stimulated B cells, followed by a translational block.

    Conclusions:

    • Small B lymphocytes function primarily as precursors, with Ig synthesis for secretion occurring upon maturation.
    • B cell maturation into Ig-secreting cells involves a significant lag period (at least 24 hours) after initial stimulation.
    • Understanding normal B cell Ig synthesis requires studying lymphoid tissues, as myeloma cells have regulatory defects.