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Two GTPs are hydrolysed on two molecules of EF-Tu for each elongation cycle during code translation
J Scoble1, N Bilgin, M Ehrenberg
1Department of Molecular Biology, University of Uppsala, Sweden.
Biochimie
|January 1, 1994
Abstract:
A new experimental design has been used to determine the number of GTPs hydrolysed per peptide bond in EF-Tu function in a poly(U)-translation system. We find that two GTPs are consumed for every amino acid incorporated into the nascent poly(Phe)-chains, in accordance with previous findings with other techniques. These results necessitate a revision of current views concerning E coli translation; also new schemes for ribosome function are discussed.