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NMR, alcohols, protein solvation and protein denaturation
1Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule-Hönggerberg, Zürich, Switzerland.
EXS
|January 1, 1994
Abstract:
The amino acid side chains of Ser, Thr and Tyr contain the hydroxyl functional group that is characteristic of alcohols, and a variety of different alcohols are used either as structure-inducing or denaturing co-solvents in physical-chemical studies of proteins. This article surveys selected aspects of the influence of protein-intrinsic and -extrinsic hydroxyl groups on studies of the solvation of peptides and proteins by nuclear magnetic resonance (NMR) spectroscopy.