Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Characteristics of the poliovirus replication complex

K Bienz¹, D Egger, T Pfister

¹Institute for Medical Microbiology, University of Basel, Switzerland.

Archives of Virology. Supplementum

|January 1, 1994

Summary

Poliovirus replication complexes (RCs) within cellular rosettes are essential for viral RNA synthesis. Intact vesicular structures are crucial for the RC

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RNA therapeutics-An evaluation of potential occupational health hazards and a strategy to establish occupational exposure limits (OELs).

Journal of occupational and environmental hygiene·2025

Same author

Preinterventional Third-Molar Assessment Using Robust Machine Learning.

Journal of dental research·2023

Same author

Controlling cleaning agent residues in pharmaceutical manufacturing: A harmonized scientific strategy.

Regulatory toxicology and pharmacology : RTP·2023

Same author

Ångstrom-Depth Resolution with Chemical Specificity at the Liquid-Vapor Interface.

Physical review letters·2023

Same author

PDE concept for controlling cleaning agent residues in pharmaceuticals- A critical analysis.

Regulatory toxicology and pharmacology : RTP·2021

Same author

Analysis of the lymphoid system in NOD/LtSz-scid IL2Rγ(null) mice engrafted with purified cord blood derived stem cells.

Journal of stem cells & regenerative medicine·2014

Area of Science:

Virology
Molecular Biology
Cell Biology

Background:

Poliovirus replication occurs within a complex structure called the replication complex (RC).
The RC is associated with virus-induced vesicles, forming a rosette-like structure within infected cells.
The RC contains viral proteins and replication intermediates, suggesting a localized site for RNA synthesis.

Purpose of the Study:

To investigate the structure and function of the poliovirus replication complex (RC) within the cellular rosette.
To determine the role of the vesicular membrane framework in poliovirus RNA replication.
To assess the in vitro activity of isolated replication complex-containing rosettes.

Main Methods:

Observation of poliovirus replication complex (RC) localization and association with vesicles in infected cells.
Characterization of the RC's central part and protruding RNA strands.
Utilizing an in vitro transcription system with isolated replication complex-containing rosettes.

Main Results:

The poliovirus replication complex (RC) is centrally located within a rosette of virus-induced vesicles.
The RC is attached to vesicular membranes, enclosing replication forks and necessary proteins.
Isolated rosettes demonstrated in vitro activity in initiation, elongation, and release of progeny RNA.
Poliovirus RNA synthesis is dependent on the structural integrity of the rosette's membraneous components.

Conclusions:

The poliovirus replication complex (RC) functions within a specialized membrane structure (rosette) that is critical for viral RNA synthesis.
The integrity of the vesicular framework is essential for the complete functionality of the poliovirus RC.
Isolated rosettes are a viable system for studying the in vitro mechanisms of poliovirus RNA replication.

Related Experiment Videos