Effect of temperature on separation of norgestrel enantiomers by high-performance liquid chromatography

The influence of mobile phase composition, concentration of beta-cyclodextrin and temperature on the high-performance liquid chromatographic separation of norgestrel was studied. In studies of the effect of temperature on the enantioselectivity of (+/-)-norgestrel, acetonitrile-water (25:75, v/v) modified by the addition of beta-cyclodextrin (14 mM) was applied as the mobile phase. Enantiomers were detected using UV detection at 240 nm. The capacity factors were measured over a wide range of column temperatures from -5 to 70 degrees C.