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Related Experiment Videos

Opioids decrease high-voltage activated calcium currents in acutely dissociated neostriatal neurons

A Stefani1, D J Surmeier, G Bernardi

  • 1Clinica Neurologica, Università di Roma Tor Vergata, Italy.

Brain Research
|April 11, 1994
PubMed
Summary
This summary is machine-generated.

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Opioid receptors in the neostriatum are located post-synaptically. Their activation by agonists like DAMGO and DPEPE modulates calcium currents in medium-sized neurons, affecting neuronal excitability.

Area of Science:

  • Neuroscience
  • Neuropharmacology

Background:

  • The patch-matrix model of neostriatal organization relies on opioid receptor distribution.
  • The precise location (pre- vs. post-synaptic) of these receptors and their functional impact on ion conductances remain unclear.

Purpose of the Study:

  • To determine if opioid receptors in the neostriatum are post-synaptically located.
  • To investigate whether opioid receptor activation modulates calcium and/or potassium conductances in neostriatal neurons.

Main Methods:

  • Acutely isolated neostriatal neurons were used to assess sensitivity to opioid receptor agonists.
  • Whole-cell patch-clamp electrophysiology was employed to measure calcium currents.

Main Results:

  • Both mu-agonist DAMGO and delta-agonist DPEPE significantly decreased whole-cell calcium currents in medium-sized neostriatal neurons at nanomolar concentrations.

Related Experiment Videos

  • These inhibitory effects on calcium currents were reversed by the opiate antagonist naloxone.
  • Potassium conductances were not explicitly mentioned as modulated in this abstract.

    • Conclusions:

    • These findings support the presence of functional, post-synaptic opioid receptors in the neostriatum.
    • Activation of these post-synaptic opioid receptors can modulate neuronal excitability by altering calcium conductances.