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In vitro antimicrobial activity of Shiva-11 against ocular pathogens
L Gunshefski1, M J Mannis, J S Cullor
1Department of Ophthalmology, School of Medicine, University of California, Davis.
Cornea
|May 1, 1994
Summary
A synthetic cecropin analog, Shiva-11, shows broad-spectrum antimicrobial activity against common ocular pathogens. This peptide demonstrates significant killing of bacteria and fungi, suggesting therapeutic potential for eye infections.
Area of Science:
- Microbiology
- Peptide Science
- Ophthalmology
Background:
- Cecropins are antimicrobial peptides from the cecropia moth with known efficacy against diverse pathogens.
- Ocular infections pose a significant threat, necessitating novel therapeutic agents.
Purpose of the Study:
- To evaluate the in vitro antimicrobial activity of the synthetic cecropin analog Shiva-11.
- To assess Shiva-11's potential against key human clinical ocular pathogens.
Main Methods:
- Shiva-11 was tested against Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, and Candida albicans.
- Antimicrobial activity was assessed by exposing bacterial suspensions to varying concentrations of Shiva-11 at 37°C.
- Colony counts were determined after 24-48 hours of incubation on nutrient agar.
Main Results:
- Shiva-11 demonstrated a greater than 3-log reduction in all tested isolates within 60 minutes.
- The compound exhibited broad-spectrum activity against gram-positive bacteria, gram-negative bacteria, and fungi.
Conclusions:
- The synthetic cecropin analog Shiva-11 possesses potent in vitro antimicrobial activity.
- Shiva-11 shows promise as a therapeutic agent for treating human clinical ocular infections.