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Updated: Aug 17, 2026

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
Published on: April 27, 2018
Mitochondrial calcium spiking: a transduction mechanism based on calcium-induced permeability transition involved in
F Ichas1, L S Jouaville, S S Sidash
1Groupe d'Etude des Systèmes Biologiques Intégrés, D(BM)2, Universitè Bordeaux II, France.
Abstract:
We report reversible Ca(2+)-induced Ca2+ release from mitochondria, which takes the form of Ca2+ spikes. Mitochondrial Ca2+ spiking is an all-or-none process with a threshold dependence on both the frequency and the amplitude of the Ca2+ pulses used as stimuli. This spiking relies on the transient operation of the mitochondrial permeability transition pore, and is initiated--in a threshold-dependent manner--with inositol-triphosphate-mediated Ca2+ responses on permeabilized cells. Evidence that mitochondrial Ca(2+)-induced Ca2+ release contributes to inositol-triphosphate-mediated Ca2+ responses in intact cells is also reported.
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