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[Evaluation of semen quality after freezing at the temperature of liquid nitrogen using a modification of two
T Ochedalski1, I Salata, A Kowalski
1I Kliniki Ginekologii Instytutu Ginekologii i Połoznictwa Akademii Medycznej, Lodzi.
Ginekologia Polska
|December 1, 1993
Abstract:
Spermatozoon survival rate was analysed after freezing at the temperature of liquid nitrogen (-196 degrees C) with the application of three different cryoprotector substances, vitelline and non-vitelline. A modified method, based on the use of sperm cells of small diameter, was introduced in freezing semen. The best results were achieved while using non-vitelline, multi-ionic cryoprotector. It was found out that glycerol alone does not protect semen from the effects of low temperature.