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Retinal adhesiveness in surgically enucleated human eyes
M F Marmor1, X Y Yao, G S Hageman
1Department of Ophthalmology, Stanford University School of Medicine, California 94305-5308.
Retina (Philadelphia, Pa.)
|January 1, 1994
Summary
Human retinal adhesion involves the interphotoreceptor matrix and is influenced by environmental factors. Cold temperatures maintain adhesion, while low pH or calcium weakens it, similar to animal models.
Area of Science:
- Ophthalmology
- Cell Biology
- Biophysics
Background:
- Retinal adhesion in animal models is linked to the interphotoreceptor matrix.
- Environmental factors like temperature, pH, and calcium concentration affect retinal adhesion.
Purpose of the Study:
- To investigate whether environmental factors modulate human retinal adhesiveness.
- To compare human retinal adhesion mechanisms with those observed in rabbits and monkeys.
Main Methods:
- Studied surgically enucleated human eyes.
- Quantified retinal adhesive strength by measuring retinal pigment epithelium adherence.
- Examined interphotoreceptor matrix components using lectin staining.
Main Results:
- Human retinal adhesiveness decreased significantly within 40 minutes post-enucleation.
- Cold temperature maintained adhesive strength, whereas low pH or calcium weakened it.
- Microscopic examination revealed stretching of interphotoreceptor matrix sheaths, indicating a bond.
Conclusions:
- Human retinal adhesion mechanisms appear similar to those in rabbits and monkeys.
- Environmental factors play a crucial role in maintaining human retinal adhesion.