Related Experiment Videos
Reconstitution of Rh (D) antigen activity from human erythrocyte membranes solubilized by deoxycholate
Science (New York, N.Y.)
|April 4, 1975
Abstract:
Proteins were selectively solubilized from human erythrocyte membranes with deoxycholate. After ultracentrifugation and preliminary fractionation procedures, the detergent was removed from the soluble membranes by Bio-Beads SM-2 and dialysis against 5 millimolar magnesium chloride. Reaggregation took place with the apparent formation of vesicles which showed serologically specific Rh antigen activity.