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Synthesis of a water-soluble serine-based neoglycolipid which can be covalently linked to solid phases
M Elofsson1, J Broddefalk, T Ekberg
1Chemical Center, University of Lund, Sweden.
Carbohydrate Research
|May 20, 1994
Abstract:
N alpha-Fmoc-serine pentafluorophenyl ester was glycosylated with perbenzoylated lactosyl bromide. The resulting product was coupled to a resin functionalized with 6-aminohexanoic acid and then N alpha-acylated to give a serine-based analogue of lactosylceramide. The water-soluble neoglycolipid was covalently linked to microtiter plates via its carboxyl group and was recognized by a lactose-binding lectin in an ELISA.