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Analysis of cocaine and metabolites in brain using solid phase extraction and full-scanning gas chromatography/ion
A Hernandez1, W Andollo, W L Hearn
1Dade County Medical Examiner Department, Miami, Florida 33136-1133.
Forensic Science International
|May 13, 1994
Abstract:
A method is described for the determination of cocaine, benzoylecgonine and cocaethylene in the human brain using Clean Screen solid phase extraction cartridges and gas chromatography/ion trap mass spectrometry with electron impact and full scan analysis. The procedure uses deuterated internal standards. Run-to-run and within-run coefficients of variation are < 7% and the sensitivity proved to be 50 ng/g from 1 g of sample. The procedure has been applied to a number of forensic cases involving cocaine intoxication. Cocaine was relatively unstable in brain tissue stored at 4 degrees C when compared to storage at -80 degrees C.