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High-performance liquid chromatographic assay for ibuprofen in whole blood using solid-phase extraction
J Sochor1, J Klimes, M Zahradnícek
1Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic.
Journal of Chromatography. B, Biomedical Applications
|April 1, 1994
Abstract:
A precise, accurate, reproducible one-step method for the high-performance chromatographic determination of ibuprofen in whole blood is described. Samples were, after haemolysis, prepared by solid-phase extraction. Analyses were performed using reversed-phase chromatography on a Separon SGX C18 column with a mobile phase of methanol-water (pH 3) and ultraviolet detection at 222 nm. The method was used for pharmacokinetic studies in rabbits.