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[Cloning and sequencing of a DNA fragment from Plasmodium falciparum FCC1/HN isolate]
1Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai.
Abstract:
A specific DNA fragment isolated from Plasmodium falciparum FCC1/HN isolate has been cloned in Bam H1 site of pUC18 and first partially sequenced by Sanger's method. The results show: G+C percent of DNA sequence is 26.8%, and the cloned DNA has many restriction sites which are conventional for subcloning. The authors suggest that this clone and sequence may be used as a guide for developing a DNA probe or PCR primers.
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