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Characterization of phospholipidic components of the natural pulmonary surfactant Curosurf
E Redenti1, T Peveri, P Ventura
1Chemical and Biopharmaceutical Department, Chiesi Farmaceutici SpA, Parma, Italy.
Farmaco (Societa Chimica Italiana : 1989)
|April 1, 1994
Abstract:
The phospholipidic classes of the natural pulmonary surfactant Curosurf were separated by bidimensional TLC and isolated. Quantitative analysis of each class was made by colorimetric determination of phosphorus. FD/MS and NH3-CI/MS were used to identify individual components. GC was used for qualitative/quantitative analysis of fatty acid moieties as methyl esters, using internal standards, by comparison with authentic samples.