[Comparative study of chemically modified hemoglobins as a basis for oxygen-carrying blood substitutes]

The series of hemoglobin derivatives were obtained with the different content of the polymer fractions; their comparative study by the methods of gel-chromatography and SDS-electrophoresis was conducted and the estimation of their functional, rheological, colloid-oncotic properties and the degree of immunogenicity was carried out in order to choose the optimal substance judging by their physico-chemical, gas-carrying and antigenic characteristics. It was shown that the polymerised hemoglobin with the polymer content of 25-30% has the optimal properties as the oxygen carrier. The 10% solution of this compound possesses oxygen-carrying characteristics identical to donors erythrocytes, viscosity of no more than 1.5 sP, colloid oncotic pressure in the range of 15-17.5 torr, it practically doesn't influence the aggregation of the formula blood elements and has low antigenicity. The results obtained allow to consider this type of poly-hemoglobin as a potential oxygen-carrying blood substitute.