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Selective isolation of poliovirus in recombinant murine cell line expressing the human poliovirus receptor gene
1Enterovirus Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
Abstract:
Sixty-eight laboratory strains representing 49 enterovirus, 10 adenovirus, and 3 reovirus serotypes were inoculated in a recombinant murine cell line expressing the human poliovirus receptor gene (L alpha cells). Only polioviruses caused cytopathic effect over a 10-day period. Likewise, only polioviruses were isolated, by use of L alpha cells, from 168 fecal specimens from children from developing countries. These results suggest that the recombinant L alpha cells can be used for selective isolation of poliovirus from clinical specimens.
Insights
L alpha cells, a special mouse cell line, selectively detect poliovirus. This method efficiently isolates poliovirus from clinical samples, aiding in disease surveillance and diagnosis.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Enteroviruses, including poliovirus, pose significant public health challenges.
- Accurate and efficient isolation of poliovirus from clinical specimens is crucial for disease control.
- Existing diagnostic methods may lack specificity or require extensive resources.
Purpose of the Study:
- To evaluate the utility of a recombinant murine cell line (L alpha cells) for the selective isolation of poliovirus.
- To assess the specificity and sensitivity of L alpha cells in detecting poliovirus in laboratory strains and clinical samples.
Main Methods:
- Inoculation of 68 laboratory strains (49 enterovirus, 10 adenovirus, 3 reovirus serotypes) into L alpha cells.
- Observation for cytopathic effects over a 10-day period.
- Isolation of viruses from 168 fecal specimens from children using L alpha cells.
Main Results:
- Only polioviruses induced a cytopathic effect in L alpha cells.
- L alpha cells successfully isolated poliovirus from clinical fecal specimens.
- The cell line demonstrated high specificity for poliovirus detection.
Conclusions:
- Recombinant L alpha cells offer a selective and effective method for poliovirus isolation.
- This cell line can be a valuable tool for diagnosing poliovirus infections.
- L alpha cells show promise for improving poliovirus surveillance in resource-limited settings.