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Low-stringency PCR with diagnostically useful primers for identification of Leptospira serovars
O L de Caballero1, E Dias Neto, M C Koury
1Centro de Pesquisas René Rachou, FIOCRUZ, Minas Gerais, Brazil.
Journal of Clinical Microbiology
|May 1, 1994
Abstract:
Primers proposed for the diagnosis of the pathogenic spirochete Leptospira spp. (C. Gravekamp, H. V. D. Kemp, M. Franzen, D. Carrington, G.J. Schoone, G.J.J.M. Van Eys, C. O. R. Everard, R.A. Hartskeel, and W.J. Terpstra, J. Gen. Microbiol. 139:1691-1700, 1993) have been found to produce complex serovar-specific patterns under low-stringency PCR conditions. Such patterns obtained by low-stringency PCR, which maintain the specific band as an internal control, offer, an approach to the standardized identification of Leptospira serovars in clinical laboratories.