Bioautographic characterization of antimicrobial substances after electrophoresis in agar gel

Bioautography following electrophoresis on agar gel, a technique for the characterization of antibiotics, is described in detail and its possible uses and advantages discussed. Eleven antibiotics of the beta-lactamic group were characterized. Penicillin G and Penicillin V showed two components with anti-microbial activity. Attempts were made to identify the components by an adaptation of the technique and the results are discussed.