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Related Experiment Videos

Specificity of human lymphocyte complement receptors

G D Ross, M J Polley

    The Journal of Experimental Medicine
    |May 1, 1975
    PubMed
    Summary
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    Researchers identified two distinct complement (C) receptors on lymphocytes. One receptor binds C4 or C3c, while the other binds C3d, demonstrating independent function and membrane localization.

    Area of Science:

    • Immunology
    • Cell Biology
    • Complement System

    Background:

    • Complement receptors play crucial roles in immune responses.
    • Previous studies identified receptors for C3b and C3d on various blood cells.
    • The existence and specificity of C4 receptors on lymphocytes required further investigation.

    Purpose of the Study:

    • To characterize the complement (C) receptor activity for C4 on human blood cells.
    • To investigate the relationship between C4 receptor activity and previously identified C3b and C3d receptors on lymphocytes.
    • To determine the distinctness and membrane localization of different complement receptors on lymphocytes.

    Main Methods:

    • Assay for inhibition of rosette formation using fluid-phase complement (C).
    • Specificity testing with various complement fragments (C3b, C3c, C3d, C4).

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  • Development and application of a double-label assay for simultaneous detection of two C receptors on individual lymphocytes.

    • Main Results:

    • Two distinct complement (C) receptors were identified on lymphocytes: an immune adherence receptor (specific for C4 or C3c) and a C3d receptor.
    • Erythrocytes share the immune adherence receptor with lymphocytes.
    • The C3d receptor showed differential binding affinities for C3b and C3d fragments, influencing rosette formation.
    • The double-label assay revealed independent capping of the two lymphocyte C receptors, indicating distinct molecular entities and independent membrane mobility.

    Conclusions:

    • Lymphocytes express at least two distinct complement (C) receptors with differing specificities for C3 and C4 fragments.
    • These receptors are located on separate molecules within the cell membrane.
    • The independent mobility of these receptors suggests distinct functional roles in immune cell interactions.