Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypertonic saline enhances cellular immune function

W G Junger1, F C Liu, W H Loomis

  • 1University of California San Diego Medical Center, Department of Surgery 92103.

Circulatory Shock
|April 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Association between long-term cumulative levels of non-high-density lipoprotein cholesterol and the risk of cardiovascular disease: a prospective cohort study].

Zhonghua yi xue za zhi·2026
Same author

Enhanced S-Factor for the ^{14}N(p,γ)^{15}O Reaction and Its Impact on the Solar Composition Problem.

Physical review letters·2025
Same author

[Consistency of claudin 18.2 expression with antibodies of different clones in gastric adenocarcinoma].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2025
Same author

[Impact of six lipid parameters on cognitive impairment in the elderly Chinese population: a prospective cohort study].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025
Same author

[Association between cumulative body mass index exposure and cognitive function among elderly people aged 60 years and older in China].

Zhonghua yi xue za zhi·2024
Same author

[Clinicopathological and molecular features of two cases of early gastric adenocarcinoma with enteroblastoid differentiation treated by endoscopic submucosal dissection].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2024
Same journal

Study of septic shock in the non-human primate: relationship of pathophysiological response to therapy with anti-TNF antibody.

Circulatory shock·1994
Same journal

Pentoxifylline inhibits mediator synthesis in an equine in vitro whole blood model of endotoxemia.

Circulatory shock·1994
Same journal

Endocrine and carbohydrate responses to interleukin-6 in vivo.

Circulatory shock·1994
Same journal

Endothelin-1 causes accumulation of leukocytes in the pulmonary circulation.

Circulatory shock·1994
Same journal

Functional requirement of the two TNF receptors for induction of apoptosis in PC60 cells and the role of mitochondria in TNF-induced cytotoxicity.

Circulatory shock·1994
Same journal

Beneficial effects of the phosphodiesterase inhibitors BRL 61063, pentoxifylline, and rolipram in a murine model of endotoxin shock.

Circulatory shock·1994
See all related articles

Hypertonic saline (HTS) boosts immune cell function in trauma patients. This resuscitation fluid may reverse post-injury immune suppression, potentially lowering sepsis risk by enhancing T-cell and monocyte activity.

Area of Science:

  • Immunology
  • Trauma Resuscitation
  • Cellular Physiology

Background:

  • Hypertonic saline (HTS) resuscitation is known to improve outcomes following trauma.
  • The impact of HTS on immune function, particularly post-traumatic immunosuppression, requires further investigation.

Purpose of the Study:

  • To investigate the effects of hypertonic saline (HTS) on human and rabbit immune cell function in vitro and in vivo.
  • To explore the potential mechanisms by which HTS influences T-cell proliferation and monocyte activity.

Main Methods:

  • In vitro studies assessed T-cell proliferation and monocyte tumor necrosis factor production using peripheral blood mononuclear cells (PBMC) from humans and rabbits exposed to varying HTS concentrations.
  • Experiments included substituting HTS with KCl to evaluate osmotic effects and measuring intracellular calcium (Ca2+i) in human PBMC.

Related Experiment Videos

  • In vivo studies in rabbits involved HTS injection to assess cell-mediated immune function via delayed-type hypersensitivity reactions.

    • Main Results:

    • HTS significantly enhanced T-cell proliferation at specific concentrations (e.g., 25 mM extracellular Na+) in both human and rabbit cells, with higher concentrations causing suppression.
    • Monocyte function, measured by tumor necrosis factor production, was augmented by up to 300% at 30 mM hypertonicity.
    • HTS increased intracellular calcium in nonstimulated human PBMC and improved cell-mediated immunity in rabbits.

    Conclusions:

    • Increased plasma osmolality, as induced by HTS, appears to up-regulate cellular immune function.
    • HTS resuscitation may counteract post-traumatic immunosuppression, potentially reducing the risk of sepsis in trauma patients.
    • The findings suggest a beneficial role for HTS in modulating the immune response to trauma.