[Effect of tissue conservation and extraction methods on the yield of extraction of follitropin]

Frozen, lyophilized or acetone-dried pituitary glands were minced and extracted with five different extraction mixtures. The glycoprotein fraction was precipitated from the extract by increasing the concentration of an appropriate solvent. It was found that the highest yield of follitropin was obtained by freezing the pituitary glands with liquid nitrogen followed by extraction with 1 M solution of ammonium chloride in 50% aqueous acetone. The following conditions were found to be optimal for obtaining high yield of extraction ratio 1:20, time of extraction 30 minutes, and the concentration of solvent for glycoprotein precipitation 80-86%.