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Factors affecting L-lactate utilization by Selenomonas ruminantium
1Department of Animal and Dairy Science, University of Georgia, Athens 30602-2771.
Journal of Animal Science
|May 1, 1994
Summary
L-lactate uptake by Selenomonas ruminantium HD4 is enhanced by L-malate and specific sodium concentrations, suggesting inducible stimulation. Proton involvement is indicated by metabolic inhibitors.
Area of Science:
- Microbiology
- Rumen microbiology
- Bacterial metabolism
Background:
- Selenomonas ruminantium is a key ruminal bacterium involved in carbohydrate fermentation.
- Understanding L-lactate utilization is crucial for ruminal function and animal nutrition.
Purpose of the Study:
- To investigate factors influencing L-lactate uptake by Selenomonas ruminantium HD4.
- To determine the effects of L-malate, sodium, pH, and other substrates on L-lactate utilization.
Main Methods:
- Measuring L-lactate uptake over time under various conditions.
- Assessing the impact of L-malate, sodium concentrations, and extracellular pH.
- Evaluating the effects of metabolic inhibitors and alternative substrates.
Main Results:
- L-malate significantly increased L-lactate uptake, particularly in lactate-grown cells, indicating inducible stimulation.
- Sodium concentrations (25-100 mM) enhanced long-term L-lactate uptake in the presence of L-malate.
- Monensin inhibited uptake, supporting the role of sodium. Proton involvement was suggested by metabolic inhibitors.
Conclusions:
- L-lactate uptake by S. ruminantium HD4 is inducible and stimulated by L-malate.
- Optimal uptake occurs at physiological rumen pH and sodium concentrations.
- Sodium and proton gradients likely play roles in L-lactate transport.