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Related Experiment Videos

A method for protein assay in Laemmli buffer

J O Karlsson1, K Ostwald, C Kåbjörn

  • 1Department of Anatomy and Cell Biology, University of Göteborg, Sweden.

Analytical Biochemistry
|May 15, 1994
PubMed
Summary

A new, cost-effective protein assay accurately measures soluble and membrane-bound proteins in Laemmli buffer using trichloroacetic acid-induced turbidity. This rapid method is suitable for quantifying proteins like bovine serum albumin in microplate formats.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Accurate protein quantification is essential in biological research.
  • Existing methods can be time-consuming or require specialized equipment.
  • Solubilizing proteins in Laemmli sodium dodecyl sulfate buffer is common for analysis.

Purpose of the Study:

  • To develop a simple, rapid, and inexpensive assay for protein quantification.
  • To adapt the assay for proteins solubilized in Laemmli buffer.
  • To determine the assay's sensitivity and applicability.

Main Methods:

  • Proteins were solubilized in Laemmli sodium dodecyl sulfate sample buffer.
  • Trichloroacetic acid was added to a final concentration of 24%.
  • Turbidity was measured at 570 nm using a microplate reader after 10-30 minutes.

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Main Results:

  • The assay demonstrated suitability for measuring bovine serum albumin (BSA).
  • Quantification range for BSA was 15 to 500 micrograms/ml in Laemmli buffer.
  • This corresponds to 2 to 75 micrograms of protein per well.

Conclusions:

  • A simple, cheap, and rapid protein assay has been developed.
  • The assay is effective for both soluble and membrane-bound proteins in Laemmli buffer.
  • Potential interference from nonproteinaceous macromolecules should be considered.