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Related Experiment Videos

Interpretive criteria for DU-6859a disk diffusion tests using 5-micrograms disks

R N Jones1, D M Johnson, M E Erwin

  • 1Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.

Diagnostic Microbiology and Infectious Disease
|February 1, 1994
PubMed
Summary

New interpretive criteria for the difluorinated quinolone DU-6859a were established using disk diffusion tests. These criteria accurately categorize bacterial susceptibility, aiding in effective antibiotic selection for clinical isolates.

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Area of Science:

  • Microbiology
  • Antimicrobial Susceptibility Testing
  • Pharmacology

Background:

  • DU-6859a is a novel difluorinated quinolone antibiotic.
  • Accurate in vitro susceptibility testing is crucial for guiding antibiotic therapy.
  • Ciprofloxacin-resistant isolates pose a significant challenge in clinical settings.

Purpose of the Study:

  • To develop and validate in vitro disk diffusion test interpretive criteria for DU-6859a.
  • To assess the performance of these criteria against a diverse range of clinical isolates, including ciprofloxacin-resistant strains.

Main Methods:

  • A disk diffusion test using 5-micrograms disks was performed on 494 clinical isolates.
  • Interpretive criteria were proposed for two minimum inhibitory concentration (MIC) breakpoints.

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  • Results were compared to determine interpretive agreement and identify potential errors.

    • Main Results:

    • Proposed criteria demonstrated high agreement (>= 97%) between different interpretive methods.
    • No major (false resistant) or very major (false susceptible) errors were observed.
    • The criteria effectively classified susceptibility for DU-6859a against tested organisms.

    Conclusions:

    • The developed disk diffusion interpretive criteria for DU-6859a are reliable and accurate.
    • These criteria can be utilized for routine susceptibility testing of DU-6859a in clinical laboratories.
    • The findings support the clinical utility of DU-6859a, particularly against strains with reduced susceptibility to other quinolones.