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Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation
H Mellor1, S R Kimball, L S Jefferson
1Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey 17033.
FEBS Letters
|August 15, 1994
Abstract:
Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF-2 alpha).