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The actions of estradiol and epidermal growth factor in endometrial and endometriotic stroma in vitro
1University of Southampton, Princess Anne Hospital, United Kingdom.
Objectives:
To compare the effects of E2 and epidermal growth factor (EGF) on DNA and protein synthesis in endometrial and endometriotic stromal cell culture.
Design:
Measurement of DNA and protein synthesis in cell cultures by trichloroacetic acid precipitation of radioactively labeled macromolecules.
Setting:
Tissue samples collected in operating theaters were processed in the cell culture laboratory.
Patients:
Samples of endometrium and endometriosis were collected synchronously at laparotomy.
Interventions:
Stromal cells were isolated from tissue samples. Cultures of endometrial and endometriotic stromal cells were treated with 0.272 ng/mL (1 nmol/L) E2 or 10 ng/mL (1.6 nmol/L) EGF.
Main Outcome Measures:
3H-Thymidine and 3H-leucine incorporation were used to assess DNA and protein synthesis respectively in cell cultures.
Results:
There was no response to 0.272 ng/mL (1 nmol/L) E2 with respect to DNA or protein synthesis in either endometrial or endometriotic stromal cells, whereas 10 ng/mL (1.6 nmol/L) EGF significantly stimulated DNA and protein synthesis in both cell types. The effect of EGF was significantly greater in endometrial stroma than in endometriotic stroma.
Conclusions:
There are differences in the stimulation of 3H-thymidine incorporation by 10 ng/mL (1.6 nmol/L) EGF in endometrial and endometriotic stromal cells in vitro.