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Related Experiment Videos

Structure and stability of human telomeric sequence

P Balagurumoorthy1, S K Brahmachari

  • 1Molecular Biophysics Unit, Indian Institute of Science, Bangalore.

The Journal of Biological Chemistry
|August 26, 1994
PubMed
Summary
This summary is machine-generated.

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Human telomeric DNA forms G-quadruplex structures. Cations and loop sequences influence these structures, which is crucial for telomere function.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Telomeric DNA, composed of tandem repeats like d(TTAGGG)n in humans, plays a critical role in chromosome stability.
  • Understanding the structural dynamics of telomeric sequences is essential for elucidating their function.

Purpose of the Study:

  • To investigate the structural properties of human telomeric repeat oligonucleotide models.
  • To characterize the G-quadruplex formation and structural polymorphism in solution.

Main Methods:

  • Circular Dichroism (CD) spectroscopy
  • Gel electrophoresis
  • Chemical probing (DEPC reactivity)

Main Results:

  • Oligonucleotides d(G3T2A)3G3 and d(T2AG3)4 formed antiparallel G-quadruplex structures via intramolecular folding.

Related Experiment Videos

  • d(G3T2AG3) adopted an antiparallel G-quadruplex structure through hairpin dimerization.
  • Adenines were located in the loops, with TTA loops oriented consistently in hairpin dimers.
  • Higher-order structures formed via stacking of G-quadruplex planes.
  • N-7 of adenine in hairpin dimers participated in Hoogsteen hydrogen bonding.
  • Partial reactivity of loop adenines with DEPC indicated structural polymorphism and varied loop orientations in d(T2AG3)4.
  • Cation type (Na+ vs. K+) affected G-quadruplex stability, loop conformation, and interactions.

    • Conclusions:

    • Telomeric G-quadruplex structures are polymorphic, with diverse loop orientations and topologies.
    • The cation, loop sequence, and number of G-tracts modulate the structure and stability of telomeric sequences.
    • These structural modulations are potentially significant for telomere function.