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Microfabrication in silicon microphysiometry

G T Baxter1, L J Bousse, T D Dawes

  • 1Molecular Devices Corp., Sunnyvale, CA 94089.

Clinical Chemistry
|September 1, 1994
PubMed
Summary
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Microphysiometry effectively detects cellular changes and bacterial antibiotic sensitivity. This technology, using light-addressable potentiometric sensors, is advancing towards high-throughput and single-cell analysis for broader applications.

Area of Science:

  • Cell Biology
  • Microfluidics
  • Biosensing Technology

Background:

  • Microphysiometry has emerged as a sensitive method for monitoring cellular physiological changes.
  • It serves as a functional assay for cellular receptor activation.
  • The technology relies on light-addressable potentiometric sensors (LAS) fabricated using photolithography and micromachining.

Purpose of the Study:

  • To demonstrate the clinical relevance of microphysiometry.
  • To apply microphysiometry for detecting bacterial antibiotic sensitivity.
  • To differentiate between bacteriostatic and bactericidal antibiotic concentrations.

Main Methods:

  • Utilized microphysiometry based on light-addressable potentiometric sensors.
  • Employed photolithography and micromachining for sensor fabrication and structural manipulation.

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  • Developed a research instrument with eight separate assay channels on a 5 cm² chip.

    • Main Results:

    • Successfully detected physiological changes in cultured cells.
    • Demonstrated the ability to assess bacterial antibiotic sensitivity.
    • Discriminated between bacteriostatic and bactericidal concentrations.
    • Presented results from an eight-channel microphysiometry instrument.

    Conclusions:

    • Microphysiometry is a clinically relevant method for assessing cellular responses and antibiotic efficacy.
    • The light-addressable potentiometric sensor platform is amenable to advanced fabrication techniques.
    • Future developments aim for high-throughput and single-cell analysis capabilities.