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Estrogens determination in urine by RP-HPLC with UV detection
S Tirendi1, T Lancetta, E Bousquet
1Istituto di Chimica Farmaceutica e Tossicologia, Universitá di Cantania, Italy.
Farmaco (Societa Chimica Italiana : 1989)
|June 1, 1994
Abstract:
A new method for the determination of estrogens by high performance liquid chromatography with UV detection at 380 nm is reported. The estrogens after extraction with Bond-Elut C18 cartridge were hydrolyzed, and derivatized with 7-chloro-4-nitro-1,3-benzoxadiazole in the presence of 18-crown-6 ether and K2CO3 and their derivatives were separated on a Hypersyl ODS 5 microns column with methanol-water (75:25 v/v) as the mobile phase. The relative standard deviation (RSD) of the method ranged from 2.1 to 3.2% and the average recovery was 98.1%.