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Updated: Aug 18, 2026

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
Published on: January 16, 2012
Simple and efficient purification of C-reactive protein from canine serum
T Onishi1, T Shimizu, T Kajikawa
1Department of Veterinary Internal Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
Abstract:
Simple purification method for canine serum, C-reactive protein (CRP) was established. By combining an affinity chromatography on Sepharose 4B coupled with o-phosphorylethanolamine and a gel filtration on a column of Sephacryl S-300, about 9 mg of purified CRP from 50 ml of canine serum was obtained. The method is simple and efficient to purify canine CRP.
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