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[Fluorescence bacterioscopy, a direct method for bacteriological food analysis]

S A Afifi, G Müller

    Die Nahrung
    |January 1, 1975
    PubMed
    Summary
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    A new fluorescence bacterioscopy method using acridine yellow offers superior detection of live and dead bacteria in food compared to traditional Gram staining, overcoming limitations in differentiating cells and detecting gram-negative bacteria.

    Area of Science:

    • Microbiology
    • Food Science
    • Microscopy

    Context:

    • Traditional bacteriological methods struggle to differentiate bacterial cells from food components.
    • Existing methods fail to detect gram-negative bacteria.
    • Accurate detection of live and dead bacteria in food is crucial for safety.

    Purpose:

    • To develop an improved method for simultaneous direct detection of live and dead bacteria in food.
    • To overcome the limitations of current bacteriological techniques.
    • To compare the efficacy of Gram's stain with fluorescence methods.

    Summary:

    • Comparative experiments used Gram's stain and fluorescence methods with various fluorochromes (primuline, auramine, acridine orange, acridine yellow).
    • Acridine yellow demonstrated superior performance over Gram's stain.

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  • A novel technique, fluorescence bacterioscopy, was developed and its pros and cons discussed.

    • Impact:

    • Provides a more effective method for bacterial detection in food matrices.
    • Enhances the ability to identify both live and dead bacterial cells, including gram-negative types.
    • Offers a valuable tool for food safety and quality control assessments.