Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between yeast TFIIIB components

J Huet1, C Conesa, N Manaud

  • 1Service de Biochimie et Génétique Moléculaire, CEA-Saclay, Gif sur Yvette, France.

Nucleic Acids Research
|August 25, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The clinical impact of frailty in fibrotic interstitial lung diseases].

Revue des maladies respiratoires·2026
Same author

Validation of a scanning technique with minimal compression for measuring muscle volume with freehand 3D ultrasound.

Journal of biomechanics·2023
Same author

[Induced abortions for maternal indications: Retrospective study in Western Normandy between 2010 and 2019].

Gynecologie, obstetrique, fertilite & senologie·2020
Same author

Intervention of the obstetrician during childbirth in a supposedly low-risk population and influence of parity.

Journal of gynecology obstetrics and human reproduction·2017
Same author

Electromagnetic modeling of large subwavelength-patterned highly resonant structures.

Optics letters·2016
Same author

Tomographic diffractive microscopy with agile illuminations for imaging targets in a noisy background.

Optics letters·2015
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Yeast transcription factor TFIIIB, essential for gene expression, is composed of TATA-binding protein (TBP) and associated factors. All TFIIIB components are crucial for transcription initiation, binding DNA, and recruiting RNA polymerase.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biochemistry

Background:

  • Yeast transcription factor TFIIIB is a crucial multi-component complex.
  • TFIIIB comprises TATA-binding protein (TBP) and associated factors TFIIIB70 and B".

Purpose of the Study:

  • To investigate the subunit interactions and functional roles of yeast TFIIIB components.
  • To elucidate the mechanism of TFIIIB in transcription initiation.

Main Methods:

  • Affinity chromatography to remove TFIIIB70.
  • Gel filtration and ion-exchange chromatography for component separation.
  • In vitro transcription assays using recombinant proteins and fractions.

Main Results:

  • TFIIIB70 can be quantitatively removed, separating TBP and B".

Related Experiment Videos

  • Weak interaction between TBP and B" observed; direct TBP binding to TFIIIB70 shown.
  • TBP is essential for basal transcription of tRNA genes.
  • TFIIIB70, TBP, and B" activity are required for TFIIIC-independent U6 RNA gene transcription.

    • Conclusions:

    • Each TFIIIB component plays a vital role in DNA binding and RNA polymerase recruitment.
    • TFIIIB functions beyond TFIIIC-dependent assembly, with essential roles for individual subunits.