[Culture of dissociated cells from cerebral hemisphere of essentially neuronal make up]

Four methods are described to obtain pure neuronal cultures from chick embryo cerebral hemispheres. In our experimental conditions the proliferation of the glial cells in inhibited and cultures of either low or high neuronal density are obtained. The isolated neurons, however, did not survive longer than 8 to 10 days; the contact with glial cells seems to be necessary for the maintenance of the neurons in long-term cultures.