Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic analyses of temperature-sensitive mutations in baculovirus late expression factors

E B Carstens1, H Chan, H Yu

  • 1Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.

Virology
|October 1, 1994
PubMed
Summary

Two temperature-sensitive mutants of Autographa californica nuclear polyhedrosis virus (AcNPV) show defects in late and very late gene expression. The lef-4 mutation impairs extracellular virus assembly and very late gene activation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vision and leadership in advancing healthcare: a conversation with Professor Justin Wu.

Hong Kong medical journal = Xianggang yi xue za zhi·2025
Same author

Complexity and weak integration promote the diversity of reef fish oral jaws.

Communications biology·2024
Same author

High-'n'-dry? A comparison of cannabis and alcohol use in drivers presenting to hospital after a vehicular collision.

Addiction (Abingdon, England)·2023
Same author

Scleritis and choroidal granuloma in a young patient with sarcoidosis.

Journal francais d'ophtalmologie·2022
Same author

Plant-Based Dietary Patterns, Body Composition, Muscle Strength and Function in Middle and Older Age: A Systematic Review.

The journal of nutrition, health & aging·2021
Same author

Idiopathic choroidal neovascularization in a young boy.

Journal francais d'ophtalmologie·2021

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Autographa californica nuclear polyhedrosis virus (AcNPV) is a baculovirus with complex gene expression stages.
  • Understanding the regulation of late and very late viral gene expression is crucial for viral replication and pathogenesis.

Purpose of the Study:

  • To characterize two temperature-sensitive mutants (ts317 and ts538) of AcNPV.
  • To investigate the role of specific genes in regulating late and very late viral gene expression and virion assembly.

Main Methods:

  • Infection of cells with AcNPV mutants at permissive and non-permissive temperatures.
  • Analysis of intracellular and extracellular viral protein levels.
  • Electron microscopy for viral morphogenesis.
  • Physical mapping and DNA sequencing of mutations.

Related Experiment Videos

  • Marker rescue and transient expression assays.

    • Main Results:

    • Mutants ts317 and ts538 showed reduced intracellular and extracellular levels of structural proteins, indicating impaired assembly or transport.
    • The ts538 mutation was mapped to the lef-4 gene, encoding a late expression factor.
    • lef-4 is essential for the activation of late genes (e.g., vp39) and very late genes (e.g., polyhedrin).
    • Viral DNA replication remained unaffected in mutant-infected cells.

    Conclusions:

    • The lef-4 gene product is critical for the activation of late and very late baculovirus gene expression.
    • A functional distinction exists between genes regulating viral DNA replication and those controlling late gene expression and virion production.
    • These findings provide insights into the regulatory mechanisms of baculovirus gene expression.