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A developmental decrease in NMDA-mediated spontaneous firing in cultured rat cerebral cortex

G J Ramakers1, C de Wit, P S Wolters

  • 1Netherlands Institute for Brain Research, Amsterdam.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
|February 1, 1993
PubMed
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Neural networks adapt to changes in activity. NMDA receptors are crucial for spontaneous brain activity in developing rat cerebral cortex cultures, influencing plasticity.

Area of Science:

  • Neuroscience
  • Developmental Neuroscience
  • Cellular Neuroscience

Background:

  • Chronic manipulation of bioelectric activity in fetal rat cerebral cortex cultures induces adaptive changes.
  • N-methyl-D-aspartate (NMDA) receptors are vital for early postnatal brain activity and in vivo activity-dependent plasticity.

Purpose of the Study:

  • To investigate the role of NMDA and non-NMDA receptors in spontaneous bioelectric activity in cultured rat cerebral cortex.
  • To examine the physiological consequences of chronic bioelectric activity suppression using tetrodotoxin.

Main Methods:

  • Assayed the effects of NMDA and non-NMDA receptor antagonists (APV and DNQX) on neuronal firing in cultured rat cerebral cortex.
  • Cultured rat cerebral cortex neurons in the presence of tetrodotoxin to suppress bioelectric activity.

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Main Results:

  • NMDA receptors were more critical than non-NMDA receptors for spontaneous activity across all ages, though their relative importance decreased over 3 weeks.
  • The NMDA antagonist APV significantly reduced burst firing, while the non-NMDA antagonist DNQX slightly increased it.
  • Chronic suppression of bioelectric activity led to increased non-variable burst firing.

Conclusions:

  • The prominent role of NMDA receptor activation in early spontaneous activity suggests their involvement in activity-dependent functional plasticity in the developing cerebral cortex.
  • Findings support adaptive mechanisms regulating functional development within neuronal networks.